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\begin{table}[htb]
	\caption{Puffer für die Reinigung mittels Ni-NTA Agarose.}
	\label{tab:IMAC}
		\centering
			\begin{tabular}{p{3.75cm}p{3.75cm}p{3.75cm}p{3.75cm}}
				\toprule
\textbf{Puffer A}       & \textbf{Puffer B}       & \textbf{Puffer C}       &\textbf{Puffer D}\\
(Äquilibrierungspuffer) & (Waschpuffer 1)         & (Waschpuffer 2)         & (Elutionspuffer)\\
\toprule
50 mM KP$_{i}$ (pH 7,4)	& 50 mM KP$_{i}$ (pH 7,4)	& 50 mM KP$_{i}$ (pH 7,4)	& 200 mM Imidazol-\\
500 mM Na-Acetat    		& 500 mM Na-Acetat    		& 20 \% Glycerol 			& Acetat (pH 7,4)\\
20 \% Glycerin 			& 20 \% Glycerin 			& 1 \% Na-Cholat			& 20 \% Glycerin\\
1 \% Na-Ccholat 			& 1 \% Na-Cholat 		& 1 \% Tween 20 		& 1 \% Na-Cholat\\
1 \% Tween 20 			& 1 \% Tween 20 		& 0,1 mM EDTA			& 1 \% Tween 20\\
0,1 mM EDTA  			& 0,1 mM EDTA   			& 0,1 mM DTE		 	& 0,1 mM EDTA\\
0,1 mM DTE 			& 0,1 mM DTE 			& 0,1 mM PMSF 			& 0,1 mM DTE\\
0,1 mM PMSF 			& 0,1 mM PMSF 			& 0,1 mM ATP			& 0,1 mM PMSF\\
					& 40 mM Imidazol  		& 40 mM Imidazol			& \\
		\bottomrule
	\end{tabular}
\end{table}

\begin{table}[htb]
	\caption{Puffer für die Reinigung mittels DEAE-Sepharose.}
	\label{tab:DEAE}
		\centering
			\begin{tabular}{p{3.75cm}p{3.75cm}p{3.75cm}p{3.75cm}}
				\toprule
\textbf{Puffer E} 			& \textbf{Puffer F} 	& \textbf{Puffer G} &\textbf{Puffer H}\\
(Äquilibrierungspuffer) & (Verdünnungspuffer) & (Waschpuffer 1) 	& (Waschpuffer 2)\\
\toprule
20 mM KP$_{i}$ (pH 7,4)	& 20 \% Glycerin     & 30 mM KP$_{i}$ (pH 7,4)	& 50 mM KP$_{i}$ (pH 7,4)\\
20 \% Glycerin 			& 1 \% Na-Cholat    & 20 \% Glycerin 		& 20 \% Glycerin\\
10 mM Imidazol 			& 0,1 mM EDTA	   & 10 mM Imidazol 		& 10 mM Imidazol\\
1 \% Na-Cholat  			& 0,1 mM DTE	   & 1 \% Na-Cholat 		& 1 \% Na-Cholat\\
0,1 \% Tween 20 		& 			   & 0,1 \% Tween 20 		& 0,1 \% Tween 20\\
0,1 mM EDTA 			&  			   & 0,1 mM EDTA 		& 0,1 mM EDTA\\
0,1 mM DTE 			&  			   & 0,1 mM DTE 			& 0,1 mM DTE\\
		\bottomrule
	\end{tabular}
\end{table}

\begin{table}[htb]
	\caption{Puffer für die Reinigung mittels SP-Sepharose.}
	\label{tab:SP}
		\centering
			\begin{tabular}{p{3.75cm}p{3.75cm}p{3.75cm}}
			\toprule
\textbf{Puffer I} 			& \textbf{Puffer J} 			& \textbf{Lagerpuffer}\\
(Waschpuffer 1) 			& (Waschpuffer 2)			&	\\
\toprule
20 mM KP$_{i}$ (pH 7,4)	& 40 mM KP$_{i}$ (pH 7,4)	& 50 mM KP$_{i}$ (pH 7,4)\\
20 \% Glycerin 			& 20 \% Glycerin					& 20 \% Glycerin\\
10 mM Imidazol 			& 10 mM Imidazol					& 1 \% Na-Cholat\\
1 \% Na-Cholat 			& 1 \% Na-Cholat					& 0,1 mM EDTA\\
0,1 mM EDTA 			& 0,1 mM EDTA					& 0,1 mM DTE\\
0,1 mM DTE 			& 0,1 mM DTE					& 0,05 \% Tween 20\\
0,1 \% Tween 20 		& 0,1 \% Tween 20				&\\				
		\bottomrule
	\end{tabular}
\end{table}

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